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ion xpress rna-seq barcode 1-16 kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ion xpress rna-seq barcode 1-16 kit
    Ion Xpress Rna Seq Barcode 1 16 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ion+xpress+rna-seq+barcode+1%E2%80%9316+kit/med_rxiv__2025__01__20__25320845-421-32-38
    Average 90 stars, based on 1 article reviews
    ion xpress rna-seq barcode 1-16 kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    RNA Library Preparation:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Adapter Ligation:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Amplification:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Purification:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    RNA Sequencing:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Software:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Recombinant:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Knock-In:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Microarray:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Control:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Sample Prep:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Library Amplification:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Transfection:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Luciferase:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Reporter Assay:

    Article Title: Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
    Article Snippet: The mRNAs were barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (Thermo Fisher Scientific), and libraries were generated using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific).

    Article Title: Metatranscriptomics by In Situ RNA Stabilization Directly and Comprehensively Revealed Episymbiotic Microbial Communities of Deep-Sea Squat Lobsters
    Article Snippet: Barcoded cDNA libraries were obtained using the Ion Total RNA-Seq kit v2 and Ion Xpress RNA-Seq Barcode 1–16 kit (Life Technologies).

    Article Title: Human-specific NOTCH2NL genes affect Notch signaling and cortical neurogenesis
    Article Snippet: Ion Xpress TM RNA-Seq Barcode 1–16 Kit , ThermoFisher , Cat#4475485.

    Article Title: ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach
    Article Snippet: In brief, the RNA library preparation involved RNA fragmentation, adapter ligation, reverse transcription and 14 cycles of PCR amplification using Ion Xpress RNA-Seq Barcode 1–16 Kit (#4475485, ThermoFisher Scientific).

    Article Title: The effect of endophytic fungi on growth and nickel accumulation in Noccaea hyperaccumulators.
    Article Snippet: After purification, the cDNA was amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, USA) and purified.

    Article Title: Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
    Article Snippet: Reverse transcription was carried out using the Ion Total RNA-seq Kit v2 and the cDNA was purified, amplified and barcoded with the Ion Xpress RNA-Seq Barcode 1–16 Kit (ThermoFisher Scientific, Waltham, MA, USA) and purified.

    Article Title: Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).

    Article Title: Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States.
    Article Snippet: Libraries were constructed using the Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) and barcoded using the Ion Xpress RNA-Seq Barcode 1–16 kit (Thermo Fisher Scientific).



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    Image Search Results


    Workflow outlining the exosomal small RNA sequencing experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small RNA transcriptome sequencing using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Workflow outlining the exosomal small RNA sequencing experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small RNA transcriptome sequencing using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Isolation, Centrifugation, Construct, Sequencing, Generated

    Read length of small RNA sequencing. Read length (nucleotide) distribution after adaptor removal. Each y-axis depicts the number of sequencing reads of all samples comprising of 5 replicates in each of the respective fraction, 3 replicates of exosomes isolated from ultracentrifugation method, and 3 replicates of GT1–7 neuronal cells. Standard deviations are shown in the bar graph.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Read length of small RNA sequencing. Read length (nucleotide) distribution after adaptor removal. Each y-axis depicts the number of sequencing reads of all samples comprising of 5 replicates in each of the respective fraction, 3 replicates of exosomes isolated from ultracentrifugation method, and 3 replicates of GT1–7 neuronal cells. Standard deviations are shown in the bar graph.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Sequencing, Isolation

    Small RNA sequencing of GT1–7 neuronal cells and GT1–7-derived exosomes isolated by 2 different protocols. (A) Circos diagram depicting small RNA transcriptome data. Track A: cytogenetic bands, chromosomes are depicted qter to pter. Track B to G: Highlight of all small RNAs (miRNA, rRNA, tRNA, piRNA, snRNA and snoRNA) identified across different sample groups in respect to GT1–7 neuronal cell, exosomes isolated from differential UC (protocol 1) and Optiprep fractions (i.e. Fraction 7 to 10) using Optiprep velocity gradient ultracentrifugation (protocol 2). (B) Distribution of small RNA species in the analyzed data sets. Profiles of different biotypes in each data set are percentage proportion to the total reads mapped to small RNA species and other elements, in particular to tRNA, rRNA, snoRNA, snRNA, piwi-RNA, miRNA, protein-coding fragments and others (i.e. repeat elements). Abbreviations: ‘miRNA’ – microRNA; ‘rRNA’ – rRNA; ‘tRNA’ – tRNA; ‘piRNA’ – piwi-interacting RNA; ‘snRNA’ – small nuclear RNA; ‘snoRNA’ – small nucleolar RNA; ‘UCexo’ – exosomes from differential UC.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Small RNA sequencing of GT1–7 neuronal cells and GT1–7-derived exosomes isolated by 2 different protocols. (A) Circos diagram depicting small RNA transcriptome data. Track A: cytogenetic bands, chromosomes are depicted qter to pter. Track B to G: Highlight of all small RNAs (miRNA, rRNA, tRNA, piRNA, snRNA and snoRNA) identified across different sample groups in respect to GT1–7 neuronal cell, exosomes isolated from differential UC (protocol 1) and Optiprep fractions (i.e. Fraction 7 to 10) using Optiprep velocity gradient ultracentrifugation (protocol 2). (B) Distribution of small RNA species in the analyzed data sets. Profiles of different biotypes in each data set are percentage proportion to the total reads mapped to small RNA species and other elements, in particular to tRNA, rRNA, snoRNA, snRNA, piwi-RNA, miRNA, protein-coding fragments and others (i.e. repeat elements). Abbreviations: ‘miRNA’ – microRNA; ‘rRNA’ – rRNA; ‘tRNA’ – tRNA; ‘piRNA’ – piwi-interacting RNA; ‘snRNA’ – small nuclear RNA; ‘snoRNA’ – small nucleolar RNA; ‘UCexo’ – exosomes from differential UC.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Derivative Assay, Isolation